Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

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RNA Polymerase Pausing during Initial Transcription

In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observ...

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ژورنال

عنوان ژورنال: Nature Communications

سال: 2018

ISSN: 2041-1723

DOI: 10.1038/s41467-018-03902-9